Class II Biosafety Cabinets (BSC)

admin — Fri, 04 Nov 2011 22:56:28 +0000

Class II Biosafety Cabinets (BSC), unlike the Class I microbiological safety cabinet (MSC) provides protection for both the user, the environment and the sample material within the cabinet’s clean air (GMP Grade A) working environment.
Protection for the user is provided by means of an advanced inflow air curtain whilst the sample material in the cabinet is protected from the dirty laboratory environment by a constant stream of hepa filtered air (airflow velocities inflow > 0.4 m/s and downflow between 0.25 and 0.5 m/sec).
The Class II BSC performance is dependent on this looped air management system of inflow and downflow air being maintained this is controlled 24/7 by a reliable fan control system ensuring optimum performance whilst giving maximum filter life for the users thus reducing life cycle costs.
Pharmatec Consult recommends either DC (Digitally Controlled) or EC (Electronically Controlled) fans which reduces power consumption with over 85% in comparison to older technology based AC fans.
There are many ways cabinet designers use to achieve basic class II MSC performance, some far more reliable and robust than others – not all class BSC are the same. Which is best?
• One-, two- or three fans?
• Two or three filters?
• Recirculating or externally ducted?
• ULPA or HEPA filtration?
• Power Consumption
• Noise
• Ergonomics
• Performance envelope
• Size
• Quality
• Price
• Certified by Porton Down, TÜV Nord, TÜV Rheinland, NSF, ACDP, DIN, LNE and BS.
It is essential that class II safety cabinets are placed in the laboratory in a suitably surveyed location within the laboratory and that wherever possible the cabinet is left uncluttered with items that will disrupt the downflow air flow. Provisions can often be made when large items have to remain in the cabinet or when, say, a microscope is to be used.
Both EN 12 469:2000 and ACDP (Advisory Committee for Dangerous Pathogens) recommend that natural gas should not be used, As this will have an impact on the cabinets airflow, the downflow filter integrity and could possibly prove to be a fire hazard.

As such this should not be offered as a standard fixture on the class II BSC.

If, after a risk assessment it is deemed necessary to have natural gas then a special gas solenoid type tap together with a low profile, foot operated ignition type burner should be used.
Seldom called a Class II Biosafety Cabinet/BSC but more commonly known amongst scientists, engineers, consultants and architects as hoods, flow hoods, safety cabinet, tissue culture cabinet and a biohazard cabinet to name a few.
Pharmatec Consult advise on a consultancy basis on any concerns a perspective purchaser may have.

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admin — Tue, 30 Aug 2011 01:17:12 +0000

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